3V0M
Crystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with a novel UDP-Gal derived inhibitor (5GW) and H-antigen acceptor
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B | Histo-blood group ABO system transferase | polymer | 298 | 34654.0 | 2 | UniProt (P16442) Pfam (PF03414) In PDB | Homo sapiens (human) | Fucosylglycoprotein 3-alpha-galactosyltransferase, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase, Glycoprotein-fucosylgalactoside alpha-galactosyltransferase, Histo-blood group A transferase, A transferase, Histo-blood group B transferase, B transferase, NAGAT, Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase soluble form |
2 | A, B | MANGANESE (II) ION | non-polymer | 54.9 | 2 | Chemie (MN) | |||
3 | A, B | 5-phenyluridine 5'-(trihydrogen diphosphate) | non-polymer | 480.3 | 2 | Chemie (5GW) | |||
4 | A, B | octyl 2-O-(6-deoxy-alpha-L-galactopyranosyl)-beta-D-galactopyranoside | non-polymer | 438.5 | 2 | Chemie (BHE) | |||
5 | B | SULFATE ION | non-polymer | 96.1 | 1 | Chemie (SO4) | |||
6 | water | water | 18.0 | 542 | Chemie (HOH) |
Sequence modifications
A, B: 64 - 354 (UniProt: P16442)
PDB | External Database | Details |
---|---|---|
Met 57 | - | expression tag |
Ala 58 | - | expression tag |
Ile 59 | - | expression tag |
Gly 60 | - | expression tag |
Glu 61 | - | expression tag |
Phe 62 | - | expression tag |
Met 63 | - | expression tag |
Gly 266 | Leu 266 | engineered mutation |
Ala 268 | Gly 268 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 2 |
Total formula weight | 69308.0 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 2043.5 | |
All* | Total formula weight | 71351.5 |