3ULQ

Crystal Structure of the Anti-Activator RapF Complexed with the Response Regulator ComA DNA Binding Domain

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Response regulator aspartate phosphatase F	polymer	383	45777.0	1	UniProt (P71002) Pfam (PF18801) Pfam (PF12895) In PDB	Bacillus subtilis
2	B	Transcriptional regulatory protein ComA	polymer	90	9974.2	1	UniProt (P14204) Pfam (PF00196) In PDB	Bacillus subtilis	Competence protein A
3	A	MANGANESE (II) ION	non-polymer		54.9	1	Chemie (MN)
4		water	water		18.0	90	Chemie (HOH)

Sequence modifications

A: 2 - 381 (UniProt: P71002)

PDB	External Database	Details
Gly -1	-	expression tag
Ser 0	-	expression tag
Val 1	-	expression tag

B: 146 - 214 (UniProt: P14204)

PDB	External Database	Details
Met 125	-	expression tag
Gly 126	-	expression tag
Ser 127	-	expression tag
Ser 128	-	expression tag
His 129	-	expression tag
His 130	-	expression tag
His 131	-	expression tag
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
Ser 135	-	expression tag
Ser 136	-	expression tag
Gly 137	-	expression tag
Leu 138	-	expression tag
Val 139	-	expression tag
Pro 140	-	expression tag
Arg 141	-	expression tag
Gly 142	-	expression tag
Ser 143	-	expression tag
His 144	-	expression tag
Met 145	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	55751.3
Non-Polymers*	Number of molecules	1
	Total formula weight	54.9
All*	Total formula weight	55806.2

*Water molecules are not included.