3UIP

Complex between human RanGAP1-SUMO1, UBC9 and the IR1 domain from RanBP2 containing IR2 Motif II

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	SUMO-conjugating enzyme UBC9	polymer	158	18138.9	1	UniProt (P63279) Pfam (PF00179)	Homo sapiens (human)	SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
2	B (B)	Small ubiquitin-related modifier 1	polymer	80	9285.5	1	UniProt (P63165) Pfam (PF11976)	Homo sapiens (human)	SUMO-1, GAP-modifying protein 1, GMP1, SMT3 homolog 3, Sentrin, Ubiquitin-homology domain protein PIC1, Ubiquitin-like protein SMT3C, Smt3C, Ubiquitin-like protein UBL1
3	C (C)	Ran GTPase-activating protein 1	polymer	171	18572.5	1	UniProt (P46060) Pfam (PF07834)	Homo sapiens (human)	RanGAP1
4	D (D)	E3 SUMO-protein ligase RanBP2	polymer	67	7803.7	1	UniProt (P49792) Pfam (PF12185)	Homo sapiens (human)	358 kDa nucleoporin, Nuclear pore complex protein Nup358, Nucleoporin Nup358, Ran-binding protein 2, RanBP2, p270
5	E, F, G, H (A, B, C, D)	water	water		18.0	343	Chemie (HOH)

Sequence modifications

C: 419 - 587 (UniProt: P46060)

PDB	External Database	Details
Ser 417	-	expression tag
Leu 418	-	expression tag

D: 2631 - 2695 (UniProt: P49792)

PDB	External Database	Details
Ser 2629	-	expression tag
Leu 2630	-	expression tag
Val 2642	Ala 2642	engineered mutation
Glu 2644	Gln 2644	engineered mutation
Lys 2647	Leu 2647	engineered mutation
Asp 2649	Thr 2649	engineered mutation
Thr 2650	Lys 2650	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	4
	Total formula weight	53800.7
All*	Total formula weight	53800.7

*Water molecules are not included.