3UBW

Complex of 14-3-3 isoform epsilon, a Mlf1 phosphopeptide and a small fragment hit from a FBDD screen

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	14-3-3 protein epsilon	polymer	261	30050.0	1	UniProt (P62258) Pfam (PF00244)	Homo sapiens (human)	14-3-3E
2	B (P)	Myeloid leukemia factor 1	polymer	14	1749.9	1	UniProt (P58340)	Homo sapiens	Myelodysplasia-myeloid leukemia factor 1
3	C, D, E, F, G (A)	TERTIARY-BUTYL ALCOHOL	non-polymer		74.1	5	Chemie (TBU)
4	H (A)	(3S)-pyrrolidin-3-ol	non-polymer		87.1	1	Chemie (6SP)
5	I, J (A, P)	water	water		18.0	152	Chemie (HOH)

Sequence modifications

A: 1 - 234 (UniProt: P62258)

PDB	External Database	Details
Met -26	-	expression tag
Ser -25	-	expression tag
Tyr -24	-	expression tag
His -23	-	expression tag
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
Asp -17	-	expression tag
Tyr -16	-	expression tag
Asp -15	-	expression tag
Ile -14	-	expression tag
Pro -13	-	expression tag
Thr -12	-	expression tag
Thr -11	-	expression tag
Glu -10	-	expression tag
Asn -9	-	expression tag
Leu -8	-	expression tag
Tyr -7	-	expression tag
Phe -6	-	expression tag
Gln -5	-	expression tag
Gly -4	-	expression tag
Ala -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	31799.9
Non-Polymers*	Number of molecules	6
	Total formula weight	457.7
All*	Total formula weight	32257.6

*Water molecules are not included.