3U8P

Cytochrome b562 integral fusion with EGFP

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Cytochrome b562 integral fusion with enhanced green fluorescent protein	polymer	349	38979.8	3	UniProt (P42212) UniProt (P0ABE7) Pfam (PF01353) Pfam (PF07361)	Aequorea victoria
2	D, E, F (A, B, C)	PROTOPORPHYRIN IX CONTAINING FE	non-polymer		616.5	3	Chemie (HEM)
3	G, H, I (A, B, C)	water	water		18.0	344	Chemie (HOH)

Sequence modifications

A, B, C: 2 - 38 (UniProt: P42212)

PDB	External Database	Details
Met 0	-	initiating methionine
Val 1	-	expression tag

A, B, C: 43 - 148 (UniProt: P0ABE7)

PDB	External Database	Details
Phe 39	-	linker
Gly 40	-	linker
Gly 41	-	linker
Ser 42	-	linker

A, B, C: 150 - 346 (UniProt: P42212)

PDB	External Database	Details
Gly 149	-	linker
Leu 174	Phe 64	engineered mutation
Cro 175	Ser 65	chromophore
Cro 175	Tyr 66	chromophore
Cro 175	Gly 67	chromophore
Leu 339	His 231	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	116939.5
Non-Polymers*	Number of molecules	3
	Total formula weight	1849.5
All*	Total formula weight	118789.0

*Water molecules are not included.