3U43

Crystal structure of the colicin E2 DNase-Im2 complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Colicin-E2 immunity protein	polymer	94	11082.2	1	UniProt (P04482) Pfam (PF01320)	Escherichia coli	ImmE2, Microcin-E2 immunity protein
2	B (B)	Colicin-E2	polymer	134	15367.4	1	UniProt (P04419) Pfam (PF21431)	Escherichia coli
3	C (B)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D (B)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
5	E, F (A, B)	water	water		18.0	319	Chemie (HOH)

Sequence modifications

A: 1 - 86 (UniProt: P04482)

PDB	External Database	Details
Leu 87	-	expression tag
Glu 88	-	expression tag
His 89	-	expression tag
His 90	-	expression tag
His 91	-	expression tag
His 92	-	expression tag
His 93	-	expression tag
His 94	-	expression tag

B: 2 - 134 (UniProt: P04419)

PDB	External Database	Details
Met 1	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	26449.6
Non-Polymers*	Number of molecules	2
	Total formula weight	105.5
All*	Total formula weight	26555.1

*Water molecules are not included.