3U0N

Crystal structure of the engineered fluorescent protein mRuby, crystal form 2

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	mRuby	polymer	232	25807.4	1	Pfam (PF01353) UniProt (by SIFTS) (Q8ISF8) In PDB	Entacmaea quadricolor (Bubble-tip anemone)	GFP-like chromoprotein
2	A	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
3		water	water		18.0	195	Chemie (HOH)

Sequence modifications

A: -4 - 227 (PDB: 3U0N)

PDB	External Database	Details
Asp -4	-	expression tag
Arg -3	-	expression tag
Trp -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Nrq 64	Met 63	chromophore
Nrq 64	Tyr 64	chromophore
Nrq 64	Gly 65	chromophore
Ser 196	Asp 196	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	25807.4
Non-Polymers*	Number of molecules	2
	Total formula weight	192.1
All*	Total formula weight	25999.6

*Water molecules are not included.