3TW7

Structure of Rhizobium etli pyruvate carboxylase T882A crystallized without acetyl coenzyme-A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Pyruvate carboxylase protein	polymer	1165	127532.0	2	UniProt (Q2K340) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785) Pfam (PF00682) Pfam (PF02436) Pfam (PF00364)	Rhizobium etli
2	C, F (A, B)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
3	D, G (A, B)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
4	E, H (A, B)	CHLORIDE ION	non-polymer		35.5	2	Chemie (CL)
5	I, J (A, B)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B: 2 - 1154 (UniProt: Q2K340)

PDB	External Database	Details
Met -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
His -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
Gly 0	-	expression tag
Gly 1	-	expression tag
Ala 882	Thr 882	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	255064.0
Non-Polymers*	Number of molecules	6
	Total formula weight	250.3
All*	Total formula weight	255314.4

*Water molecules are not included.