3TW3

Crystal structure of RtcA.ATP.Co ternary complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	RNA 3'-terminal phosphate cyclase	polymer	358	38304.5	1	UniProt (P46849) Pfam (PF01137) Pfam (PF05189)	Escherichia coli	RNA cyclase, RNA-3'-phosphate cyclase
2	B (A)	ADENOSINE-5'-TRIPHOSPHATE	non-polymer		507.2	1	Chemie (ATP)
3	C, D, E (A)	COBALT (II) ION	non-polymer		58.9	3	Chemie (CO)
4	F (A)	SULFATE ION	non-polymer		96.1	1	Chemie (SO4)
5	G (A)	water	water		18.0	140	Chemie (HOH)

Sequence modifications

A: 2 - 339 (UniProt: P46849)

PDB	External Database	Details
Gly -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
Ser -7	-	expression tag
Ser -6	-	expression tag
Gly -5	-	expression tag
His -4	-	expression tag
Ile -3	-	expression tag
Glu -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag
His 1	-	expression tag
Ser 308	Cys 307	engineered mutation
Asn 309	His 308	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38304.5
Non-Polymers*	Number of molecules	5
	Total formula weight	780.0
All*	Total formula weight	39084.6

*Water molecules are not included.