3TUI

Inward facing conformations of the MetNI methionine ABC transporter: CY5 native crystal form

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, E, F	D-methionine transport system permease protein metI	polymer	217	23269.9	4	UniProt (P31547) Pfam (PF00528) In PDB	Escherichia coli
2	C, D, G, H	Methionine import ATP-binding protein MetN	polymer	366	40610.2	4	UniProt (P30750) Pfam (PF00005) Pfam (PF09383) In PDB	Escherichia coli
3	C, D, G, H	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	4	Chemie (ADP)
4		water	water		18.0	146	Chemie (HOH)

Sequence modifications

C, D, G, H: 1 - 343 (UniProt: P30750)

PDB	External Database	Details
Met -22	-	expression tag
Gly -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
Ser -10	-	expression tag
Ser -9	-	expression tag
Gly -8	-	expression tag
His -7	-	expression tag
Ile -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
His 0	-	expression tag
Gln 166	Glu 166	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	8
	Total formula weight	255520.6
Non-Polymers*	Number of molecules	4
	Total formula weight	1708.8
All*	Total formula weight	257229.4

*Water molecules are not included.