3TU4

Crystal structure of the Sir3 BAH domain in complex with a nucleosome core particle.

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, E (A, E)	Histone H3.2	polymer	135	15303.9	2	UniProt (P84233) Pfam (PF00125)	Xenopus laevis (clawed frog common platanna platanna)
2	B, F (B, F)	Histone H4	polymer	102	11263.2	2	UniProt (P62799) Pfam (PF15511)	Xenopus laevis (clawed frog common platanna platanna)
3	C, G (C, G)	Histone H2A	polymer	129	13978.2	2	UniProt (Q6AZJ8) Pfam (PF00125) Pfam (PF16211) UniProt (by SIFTS) (P06897)	Xenopus laevis (clawed frog common platanna platanna)
4	D, H (D, H)	Histone H2B 1.1	polymer	122	13524.8	2	UniProt (P02281) Pfam (PF00125)	Xenopus laevis (clawed frog common platanna platanna)	H2B1.1
5	I (I)	DNA (146-MER)	polymer	147	45137.8	1
6	J (J)	DNA (146-MER)	polymer	147	45609.1	1
7	K, L (K, L)	Regulatory protein SIR3	polymer	229	27263.9	2	UniProt (P06701) Pfam (PF01426)	Saccharomyces cerevisiae S288c (Baker's yeast)	Silent information regulator 3

Sequence modifications

A, E: 1 - 135 (UniProt: P84233)

PDB	External Database	Details
Ala 102	Gly 103	conflict

D, H: 1 - 122 (UniProt: P02281)

PDB	External Database	Details
Thr 29	Ser 33	conflict

K, L: 1 - 213 (UniProt: P06701)

PDB	External Database	Details
Asn 205	Asp 205	engineered mutation
Ser 214	-	expression tag
Val 215	-	expression tag
Asp 216	-	expression tag
Lys 217	-	expression tag
Leu 218	-	expression tag
Ala 219	-	expression tag
Ala 220	-	expression tag
Ala 221	-	expression tag
Leu 222	-	expression tag
Glu 223	-	expression tag
His 224	-	expression tag
His 225	-	expression tag
His 226	-	expression tag
His 227	-	expression tag
His 228	-	expression tag
His 229	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	253415.0
All*	Total formula weight	253415.0

*Water molecules are not included.