3TT3
Crystal Structure of LeuT in the inward-open conformation in complex with Fab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Leucine transporter LeuT | polymer | 519 | 57909.3 | 1 | UniProt (O67854) Pfam (PF00209) | Aquifex aeolicus | |
| 2 | B (L) | mouse monoclonal 1gG1 Fab fragment, heavy chain | polymer | 218 | 24208.6 | 1 | Mus musculus | ||
| 3 | C (H) | mouse monoclonal 1gG1 Fab fragment, kappa light chain | polymer | 221 | 23985.9 | 1 | Mus musculus | ||
| 4 | D (A) | octyl 1-thio-beta-D-glucopyranoside | non-polymer | 308.4 | 1 | Chemie (SOG) |
Sequence modifications
A: 1 - 513 (UniProt: O67854)
| PDB | External Database | Details |
|---|---|---|
| Ala 268 | Tyr 268 | engineered mutation |
| Ala 288 | Lys 288 | engineered mutation |
| Val 354 | Thr 354 | engineered mutation |
| Ala 355 | Ser 355 | engineered mutation |
| Gly 514 | - | expression tag |
| Thr 515 | - | expression tag |
| Leu 516 | - | expression tag |
| Val 517 | - | expression tag |
| Pro 518 | - | expression tag |
| Arg 519 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 106103.8 | |
| Non-Polymers* | Number of molecules | 1 |
| Total formula weight | 308.4 | |
| All* | Total formula weight | 106412.2 |
