3TT1

Crystal Structure of LeuT in the outward-open conformation in complex with Fab

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Leucine transporter LeuT	polymer	519	58003.3	2	UniProt (O67854) Pfam (PF00209)	Aquifex aeolicus
2	C, E (L, M)	mouse monoclonal 1gG2a Fab fragment, heavy chain	polymer	218	23911.1	2		Mus musculus (mouse)
3	D, F (H, I)	mouse monoclonal 1gG2a Fab fragment, kappa light chain	polymer	219	23575.4	2		Mus musculus
4	G, H, L, M (A, B)	SODIUM ION	non-polymer		23.0	4	Chemie (NA) PubChem (923)
5	I, J, K, N, O (A, B)	octyl 1-thio-beta-D-glucopyranoside	non-polymer		308.4	5	Chemie (SOG) PubChem (20765008) PubChem (22840433) PubChem (3035440) PubChem (24802438) PubChem (24802439) PubChem (363418) PubChem (44120068) PubChem (446404) PubChem (46782626) PubChem (656909) PubChem (53720324) PubChem (53720325) PubChem (53720326) PubChem (53720327) PubChem (89711063) PubChem (92151891) PubChem (95858717) PubChem (95858718) PubChem (96327603) PubChem (96367497) PubChem (96367498) PubChem (98118655) PubChem (97944776) PubChem (101682121) PubChem (102041106) PubChem (124524845) PubChem (171395924)
6	P, Q, R (A, B, H)	water	water		18.0	28	Chemie (HOH)

Sequence modifications

A, B: 1 - 513 (UniProt: O67854)

PDB	External Database	Details
Phe 108	Tyr 108	engineered mutation
Ala 288	Lys 288	engineered mutation
Gly 514	-	expression tag
Thr 515	-	expression tag
Leu 516	-	expression tag
Val 517	-	expression tag
Pro 518	-	expression tag
Arg 519	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	210979.7
Non-Polymers*	Number of molecules	9
	Total formula weight	1634.1
All*	Total formula weight	212613.8

*Water molecules are not included.