3TT1
Crystal Structure of LeuT in the outward-open conformation in complex with Fab
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Leucine transporter LeuT | polymer | 519 | 58003.3 | 2 | UniProt (O67854) Pfam (PF00209) | Aquifex aeolicus | |
| 2 | C, E (L, M) | mouse monoclonal 1gG2a Fab fragment, heavy chain | polymer | 218 | 23911.1 | 2 | Mus musculus (mouse) | ||
| 3 | D, F (H, I) | mouse monoclonal 1gG2a Fab fragment, kappa light chain | polymer | 219 | 23575.4 | 2 | Mus musculus | ||
| 4 | G, H, L, M (A, B) | SODIUM ION | non-polymer | 23.0 | 4 | Chemie (NA) | |||
| 5 | I, J, K, N, O (A, B) | octyl 1-thio-beta-D-glucopyranoside | non-polymer | 308.4 | 5 | Chemie (SOG) | |||
| 6 | P, Q, R (A, B, H) | water | water | 18.0 | 28 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 513 (UniProt: O67854)
| PDB | External Database | Details |
|---|---|---|
| Phe 108 | Tyr 108 | engineered mutation |
| Ala 288 | Lys 288 | engineered mutation |
| Gly 514 | - | expression tag |
| Thr 515 | - | expression tag |
| Leu 516 | - | expression tag |
| Val 517 | - | expression tag |
| Pro 518 | - | expression tag |
| Arg 519 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 210979.7 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1634.1 | |
| All* | Total formula weight | 212613.8 |
