3TS8

Crystal structure of a multidomain human p53 tetramer bound to the natural CDKN1A(p21) p53-response element

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D	Cellular tumor antigen p53	polymer	234	26618.1	4	UniProt (P04637) Pfam (PF00870) Pfam (PF07710) In PDB	Homo sapiens (human)	Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, p53CR2
2	K	CDKN1A(p21) sense strand	polymer	26	8029.2	1
3	L	CDKN1A(p21) anti-sense strand	polymer	26	7944.1	1
4	A, B, C, D	ZINC ION	non-polymer		65.4	4	Chemie (ZN)
5		water	water		18.0	105	Chemie (HOH)

Sequence modifications

A, B, C, D: 94 - 291 (UniProt: P04637)

PDB	External Database	Details
Val 135	Cys 135	engineered mutation
Val 141	Cys 141	engineered mutation
Tyr 146	Trp 146	engineered mutation
Ser 182	Cys 182	engineered mutation
Ala 203	Val 203	engineered mutation
Pro 209	Arg 209	engineered mutation
Tyr 229	Cys 229	engineered mutation
Tyr 233	His 233	engineered mutation
Phe 234	Tyr 234	engineered mutation
Lys 235	Asn 235	engineered mutation
Phe 236	Tyr 236	engineered mutation
Val 253	Thr 253	engineered mutation
Asp 268	Asn 268	engineered mutation

A, B, C, D: 321 - 356 (UniProt: P04637)

PDB	External Database	Details
Thr 322	Pro 322	engineered mutation
Met 323	Leu 323	engineered mutation
Gln 340	Met 340	engineered mutation
Arg 344	Leu 344	engineered mutation
Thr 356	Gly 356	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	122445.7
Non-Polymers*	Number of molecules	4
	Total formula weight	261.6
All*	Total formula weight	122707.4

*Water molecules are not included.