3TRO

Crystal Structure of Leishmania major dihydroorotate dehydrogenase mutant D171A

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Dihydroorotate dehydrogenase	polymer	354	38201.6	2	UniProt (Q4QEW7) Pfam (PF01180)	Leishmania major
2	C, J (A, B)	FLAVIN MONONUCLEOTIDE	non-polymer		456.3	2	Chemie (FMN)
3	D, E (A)	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
4	F, G, H, I, K... (A, B)	GLYCEROL	non-polymer		92.1	8	Chemie (GOL)
5	O, P (A, B)	water	water		18.0	433	Chemie (HOH)

Sequence modifications

A, B: 1 - 320 (UniProt: Q4QEW7)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Ala 171	Asp 171	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76403.3
Non-Polymers*	Number of molecules	12
	Total formula weight	1841.6
All*	Total formula weight	78244.8

*Water molecules are not included.