3TNX

Structure of the precursor of a thermostable variant of papain at 2.6 Angstroem resolution

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Papain	polymer	363	41067.6	2	UniProt (P00784) Pfam (PF08246) Pfam (PF00112)	Carica papaya (mamon)	Papaya proteinase I, PPI
2	C (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	D, E (A, C)	water	water		18.0	133	Chemie (HOH)

Sequence modifications

A, C: 1 - 319 (UniProt: P00784)

PDB	External Database	Details
Met -43	-	expression tag
His -42	-	expression tag
His -41	-	expression tag
His -40	-	expression tag
His -39	-	expression tag
His -38	-	expression tag
His -37	-	expression tag
Ser -36	-	expression tag
Ser -35	-	expression tag
Gly -34	-	expression tag
Leu -33	-	expression tag
Val -32	-	expression tag
Pro -31	-	expression tag
Arg -30	-	expression tag
Gly -29	-	expression tag
Ser -28	-	expression tag
Gly -27	-	expression tag
Met -26	-	expression tag
Lys -25	-	expression tag
Glu -24	-	expression tag
Thr -23	-	expression tag
Ala -22	-	expression tag
Ala -21	-	expression tag
Ala -20	-	expression tag
Lys -19	-	expression tag
Phe -18	-	expression tag
Glu -17	-	expression tag
Arg -16	-	expression tag
Gln -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Asp -12	-	expression tag
Ser -11	-	expression tag
Pro -10	-	expression tag
Asp -9	-	expression tag
Leu -8	-	expression tag
Gly -7	-	expression tag
Thr -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Met 0	-	expression tag
Ala 132	Cys 158	engineered mutation
Ser 139	Val 165	engineered mutation
Ser 143	Gly 169	engineered mutation
Arg 281	Lys 307	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	82135.1
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	82170.6

*Water molecules are not included.