3TNB

Crystal structure of GkaP mutant G209D/R230H from Geobacillus kaustophilus HTA426

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Phosphotriesterase	polymer	360	40075.5	2	UniProt (Q5KZU5) Pfam (PF02126) In PDB	Geobacillus kaustophilus
2	A, B	COBALT (II) ION	non-polymer		58.9	4	Chemie (CO)
3		water	water		18.0	709	Chemie (HOH)

Sequence modifications

A, B: 1 - 326 (UniProt: Q5KZU5)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Leu -20	-	expression tag
Val -19	-	expression tag
Pro -18	-	expression tag
Arg -17	-	expression tag
Gly -16	-	expression tag
Ser -15	-	expression tag
His -14	-	expression tag
Met -13	-	expression tag
Ala -12	-	expression tag
Ser -11	-	expression tag
Met -10	-	expression tag
Thr -9	-	expression tag
Gly -8	-	expression tag
Gly -7	-	expression tag
Gln -6	-	expression tag
Gln -5	-	expression tag
Met -4	-	expression tag
Gly -3	-	expression tag
Arg -2	-	expression tag
Gly -1	-	expression tag
Ser 0	-	expression tag
Asp 209	Gly 209	engineered mutation
His 230	Arg 230	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	80150.9
Non-Polymers*	Number of molecules	4
	Total formula weight	235.7
All*	Total formula weight	80386.7

*Water molecules are not included.