3TKA

crystal structure and solution saxs of methyltransferase rsmh from E.coli

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Ribosomal RNA small subunit methyltransferase H	polymer	347	38664.9	1	UniProt (P60390) Pfam (PF01795)	Escherichia coli	16S rRNA m(4)C1402 methyltransferase, rRNA (cytosine-N(4)-)-methyltransferase RsmH
2	B (A)	S-ADENOSYLMETHIONINE	non-polymer		398.4	1	Chemie (SAM)
3	C (A)	4-AMINO-1-BETA-D-RIBOFURANOSYL-2(1H)-PYRIMIDINONE	non-polymer		243.2	1	Chemie (CTN)
4	D, E, F, G, H (A)	SULFATE ION	non-polymer		96.1	5	Chemie (SO4)
5	I (A)	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
6	J (A)	water	water		18.0	145	Chemie (HOH)

Sequence modifications

A: 1 - 313 (UniProt: P60390)

PDB	External Database	Details
Met -33	-	expression tag
Gly -32	-	expression tag
Ser -31	-	expression tag
Ser -30	-	expression tag
His -29	-	expression tag
His -28	-	expression tag
His -27	-	expression tag
His -26	-	expression tag
His -25	-	expression tag
His -24	-	expression tag
Ser -23	-	expression tag
Ser -22	-	expression tag
Gly -21	-	expression tag
Glu -20	-	expression tag
Asn -19	-	expression tag
Leu -18	-	expression tag
Tyr -17	-	expression tag
Phe -16	-	expression tag
Glu -15	-	expression tag
Gly -14	-	expression tag
Ser -13	-	expression tag
His -12	-	expression tag
Met -11	-	expression tag
Ala -10	-	expression tag
Ser -9	-	expression tag
Met -8	-	expression tag
Thr -7	-	expression tag
Gly -6	-	expression tag
Gly -5	-	expression tag
Gln -4	-	expression tag
Gln -3	-	expression tag
Met -2	-	expression tag
Gly -1	-	expression tag
Arg 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	38664.9
Non-Polymers*	Number of molecules	8
	Total formula weight	1316.2
All*	Total formula weight	39981.1

*Water molecules are not included.