3THV
Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddATP-dT in Closed Conformation
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, D | DNA polymerase I | polymer | 592 | 67490.3 | 2 | UniProt (C9RTX7) In PDB | Geobacillus | |
2 | B, E | 5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(2DA))-3' | polymer | 9 | 2659.8 | 2 | |||
3 | C, F | 5'-D(*C*AP*TP*TP*TP*GP*AP*GP*TP*CP*AP*GP*G)-3' | polymer | 13 | 4006.6 | 2 | |||
4 | A, D | 2',3'-dideoxyadenosine triphosphate | non-polymer | 475.2 | 2 | Chemie (DDS) | |||
5 | A, D | MAGNESIUM ION | non-polymer | 24.3 | 2 | Chemie (MG) | |||
6 | A, D | SULFATE ION | non-polymer | 96.1 | 3 | Chemie (SO4) | |||
7 | water | water | 18.0 | 1696 | Chemie (HOH) |
Sequence modifications
A, D: 285 - 876 (UniProt: C9RTX7)
PDB | External Database | Details |
---|---|---|
Glu 456 | Ala 456 | SEE REMARK 999 |
Lys 505 | Glu 505 | SEE REMARK 999 |
Ala 598 | Asp 598 | engineered mutation |
Tyr 710 | Phe 710 | engineered mutation |
His 823 | Arg 823 | SEE REMARK 999 |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 6 |
Total formula weight | 148313.4 | |
Non-Polymers* | Number of molecules | 7 |
Total formula weight | 1287.2 | |
All* | Total formula weight | 149600.5 |