3THV

Crystal Structure of Bacillus DNA Polymerase I Large Fragment Bound to DNA and ddATP-dT in Closed Conformation

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, D)	DNA polymerase I	polymer	592	67490.3	2	UniProt (C9RTX7)	Geobacillus
2	C, E (B, E)	5'-D(*CP*CP*TP*GP*AP*CP*TP*CP*(2DA))-3'	polymer	9	2659.8	2
3	D, F (C, F)	5'-D(*C*AP*TP*TP*TP*GP*AP*GP*TP*CP*AP*GP*G)-3'	polymer	13	4006.6	2
4	G, J (A, D)	2',3'-dideoxyadenosine triphosphate	non-polymer		475.2	2	Chemie (DDS)
5	H, K (A, D)	MAGNESIUM ION	non-polymer		24.3	2	Chemie (MG)
6	I, L, M (A, D)	SULFATE ION	non-polymer		96.1	3	Chemie (SO4)
7	N, O, P, Q, R... (A, D, B, C, E...)	water	water		18.0	1696	Chemie (HOH)

Sequence modifications

A, D: 285 - 876 (UniProt: C9RTX7)

PDB	External Database	Details
Glu 456	Ala 456	SEE REMARK 999
Lys 505	Glu 505	SEE REMARK 999
Ala 598	Asp 598	engineered mutation
Tyr 710	Phe 710	engineered mutation
His 823	Arg 823	SEE REMARK 999

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	148313.4
Non-Polymers*	Number of molecules	7
	Total formula weight	1287.2
All*	Total formula weight	149600.5

*Water molecules are not included.