3TE9

1.8 Angstrom Resolution Crystal Structure of K135M Mutant of Transaldolase B (TalA) from Francisella tularensis in Complex with Fructose 6-phosphate

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Transaldolase	polymer	345	38478.1	2	UniProt (Q5NFX0) Pfam (PF00923) In PDB	Francisella tularensis subsp. tularensis
2	A	FRUCTOSE -6-PHOSPHATE	non-polymer		260.1	1	Chemie (F6R)
3	A, B	DI(HYDROXYETHYL)ETHER	non-polymer		106.1	2	Chemie (PEG)
4	A, B	TRIETHYLENE GLYCOL	non-polymer		150.2	6	Chemie (PGE)
5	B	PHOSPHATE ION	non-polymer		95.0	1	Chemie (PO4)
6		water	water		18.0	610	Chemie (HOH)

Sequence modifications

A, B: 1 - 321 (UniProt: Q5NFX0)

PDB	External Database	Details
Met -23	-	initiating methionine
His -22	-	expression tag
His -21	-	expression tag
His -20	-	expression tag
His -19	-	expression tag
His -18	-	expression tag
His -17	-	expression tag
Ser -16	-	expression tag
Ser -15	-	expression tag
Gly -14	-	expression tag
Val -13	-	expression tag
Asp -12	-	expression tag
Leu -11	-	expression tag
Gly -10	-	expression tag
Thr -9	-	expression tag
Glu -8	-	expression tag
Asn -7	-	expression tag
Leu -6	-	expression tag
Tyr -5	-	expression tag
Phe -4	-	expression tag
Gln -3	-	expression tag
Ser -2	-	expression tag
Asn -1	-	expression tag
Ala 0	-	expression tag
Met 135	Lys 135	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	76956.2
Non-Polymers*	Number of molecules	10
	Total formula weight	1468.4
All*	Total formula weight	78424.6

*Water molecules are not included.