3TDZ
N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex: Structure of a human Cul1WHB-Dcn1P-stapled acetylated Ubc12N complex
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, C (A, B) | DCN1-like protein 1 | polymer | 200 | 23237.5 | 2 | UniProt (Q96GG9) Pfam (PF03556) | Homo sapiens (human) | DCUN1 domain-containing protein 1, Defective in cullin neddylation protein 1-like protein 1, Squamous cell carcinoma-related oncogene |
| 2 | B, D (C, D) | Cullin-1 | polymer | 77 | 9015.7 | 2 | UniProt (Q13616) Pfam (PF10557) | Homo sapiens (human) | CUL-1 |
| 3 | E, F (E, F) | STAPLED PEPTIDE | polymer | 13 | 1547.9 | 2 | UniProt (P61081) BIRD (PRD_001077) | Homo sapiens | NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M |
| 4 | G, H, I, J, K... (A, C, B, D, E...) | water | water | 18.0 | 485 | Chemie (HOH) |
Sequence modifications
A, B: 62 - 259 (UniProt: Q96GG9)
C, D: 702 - 776 (UniProt: Q13616)
E, F: 2 - 12 (UniProt: P61081)
| PDB | External Database | Details |
|---|---|---|
| Gly 60 | - | expression tag |
| Ser 61 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Gly 700 | - | expression tag |
| Ser 701 | - | expression tag |
| PDB | External Database | Details |
|---|---|---|
| Ace 0 | - | acetylation |
| Mse 1 | - | initiating methionine |
| Mk8 5 | Phe 5 | engineered mutation |
| Mk8 9 | Gln 9 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 67602.1 | |
| All* | Total formula weight | 67602.1 |
