3TDU

N-terminal acetylation acts as an avidity enhancer within an interconnected multiprotein complex: Structure of a human Cul1WHB-Dcn1P-acetylated Ubc12N complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	DCN1-like protein 1	polymer	200	23237.5	2	UniProt (Q96GG9) Pfam (PF03556)	Homo sapiens (human)	DCUN1 domain-containing protein 1, Defective in cullin neddylation protein 1-like protein 1, Squamous cell carcinoma-related oncogene
2	C, D (C, D)	Cullin-1	polymer	77	9015.7	2	UniProt (Q13616) Pfam (PF10557)	Homo sapiens (human)	CUL-1
3	E, F (E, F)	NEDD8-conjugating enzyme Ubc12	polymer	16	1909.3	2	UniProt (P61081)	HOMO SAPIENS	NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M
4	G, H, I, J, K... (A, B, C, D, E...)	water	water		18.0	589	Chemie (HOH)

Sequence modifications

A, B: 62 - 259 (UniProt: Q96GG9)

PDB	External Database	Details
Gly 60	-	expression tag
Ser 61	-	expression tag

C, D: 702 - 776 (UniProt: Q13616)

PDB	External Database	Details
Gly 700	-	expression tag
Ser 701	-	expression tag

E, F: 3 - 16 (UniProt: P61081)

PDB	External Database	Details
Ace 1	-	acetylation
Met 2	-	initiating methionine

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	68324.9
All*	Total formula weight	68324.9

*Water molecules are not included.