3TCM

Crystal Structure of Alanine Aminotransferase from Hordeum vulgare

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Alanine aminotransferase 2	polymer	500	55203.5	2	UniProt (P52894) Pfam (PF00155) In PDB	Hordeum vulgare (barley)	ALAAT-2, Glutamate pyruvate transaminase 2, GPT, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2
2	A, B	D-[3-HYDROXY-2-METHYL-5-PHOSPHONOOXYMETHYL-PYRIDIN-4-YLMETHYL]-N,O-CYCLOSERYLAMIDE	non-polymer		333.2	2	Chemie (DCS)
3		water	water		18.0	61	Chemie (HOH)

Sequence modifications

A, B: 1 - 482 (UniProt: P52894)

PDB	External Database	Details
Met -17	-	initiating methionine
His -16	-	expression tag
His -15	-	expression tag
His -14	-	expression tag
His -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Gly -6	-	expression tag
Thr -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Asp -1	-	expression tag
Lys 0	-	expression tag
His 119	Lys 119	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	110407.1
Non-Polymers*	Number of molecules	2
	Total formula weight	666.5
All*	Total formula weight	111073.5

*Water molecules are not included.