3TAF

5-fluorocytosine paired with ddGMP in RB69 gp43

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, D, G, J (A, B, C, D)	DNA-directed DNA polymerase	polymer	906	105069.6	4	UniProt (Q38087) Pfam (PF03104) Pfam (PF00136)	Escherichia phage RB69	Gp43
2	B, E, H, K (E, G, I, K)	DNA (5'-D(*CP*CP*(C37)P*GP*GP*TP*AP*TP*GP*AP*CP*AP*GP*CP*CP*GP*CP*G)-3')	polymer	18	5520.5	4		Synthetic construct
3	C, F, I, L (F, H, J, L)	DNA (5'-D(*GP*CP*GP*GP*CP*TP*GP*TP*CP*AP*TP*AP*CP*CP*G)-3')	polymer	15	4586.0	4		Synthetic construct
4	M, N, O, P (E, G, I, K)	SULFATE ION	non-polymer		96.1	4	Chemie (SO4)
5	AA, BA, Q, R, S... (K, L, A, E, F...)	water	water		18.0	120	Chemie (HOH)

Sequence modifications

A, B, C, D: 1 - 903 (UniProt: Q38087)

PDB	External Database	Details
Ala 222	Asp 222	engineered mutation
Ala 327	Asp 327	engineered mutation
His 904	-	expression tag
His 905	-	expression tag
His 906	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	12
	Total formula weight	460704.4
Non-Polymers*	Number of molecules	4
	Total formula weight	384.3
All*	Total formula weight	461088.7

*Water molecules are not included.