3T53

Crystal structures of the extrusion state of the CusBA adaptor-transporter complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (B, C)	Cation efflux system protein CusB	polymer	336	36615.7	2	UniProt (P77239) Pfam (PF00529) Pfam (PF16572)	Escherichia coli
2	C (A)	Cation efflux system protein CusA	polymer	1054	115702.8	1	UniProt (P38054) Pfam (PF00873)	Escherichia coli
3	D (A)	COPPER (II) ION	non-polymer		63.5	1	Chemie (CU)
4	E, F, G (B, C, A)	water	water		18.0	21	Chemie (HOH)

Sequence modifications

B, C: 78 - 407 (UniProt: P77239)

PDB	External Database	Details
His 408	-	expression tag
His 409	-	expression tag
His 410	-	expression tag
His 411	-	expression tag
His 412	-	expression tag
His 413	-	expression tag

A: 1 - 1047 (UniProt: P38054)

PDB	External Database	Details
Gly -6	-	expression tag
His -5	-	expression tag
His -4	-	expression tag
His -3	-	expression tag
His -2	-	expression tag
His -1	-	expression tag
His 0	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	188934.1
Non-Polymers*	Number of molecules	1
	Total formula weight	63.5
All*	Total formula weight	188997.6

*Water molecules are not included.