3T52
L29I Mutation in an Aryl Esterase from Pseudomonas fluorescens Leads to Unique Peptide Flip and Increased Activity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Arylesterase | polymer | 271 | 29994.0 | 6 | UniProt (P22862) Pfam (PF00561) | Pseudomonas fluorescens | Aryl-ester hydrolase, PFE, bromoperoxidase |
| 2 | AB, AC, BB, CB, DB... (C, E, D, A, B...) | GLYCEROL | non-polymer | 92.1 | 32 | Chemie (GOL) | |||
| 3 | EB, H, LC, MA, MC... (D, A, F, C, E...) | SULFATE ION | non-polymer | 96.1 | 12 | Chemie (SO4) | |||
| 4 | FB, I, J, PA, QA... (D, A, C, E, B) | CHLORIDE ION | non-polymer | 35.5 | 7 | Chemie (CL) | |||
| 5 | AA, BA, BC, CA, HC... (B, E, D, A, F...) | HYDROGEN PEROXIDE | non-polymer | 34.0 | 19 | Chemie (PEO) | |||
| 6 | CC, DA, DC, EA, EC... (E, B, D, A, C...) | ACETATE ION | non-polymer | 59.0 | 28 | Chemie (ACT) | |||
| 7 | AD, BD, CD, DD, ED... (A, B, C, D, E...) | water | water | 18.0 | 1166 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 271 (UniProt: P22862)
| PDB | External Database | Details |
|---|---|---|
| Ile 29 | Leu 30 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 179963.7 | |
| Non-Polymers* | Number of molecules | 98 |
| Total formula weight | 6647.5 | |
| All* | Total formula weight | 186611.2 |
