3T4U
L29I Mutation in an Aryl Esterase from Pseudomonas fluorescens Leads to Unique Peptide Flip and Increased Activity
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Arylesterase | polymer | 271 | 29994.0 | 6 | UniProt (P22862) Pfam (PF00561) | Pseudomonas fluorescens | Aryl-ester hydrolase, PFE, bromoperoxidase |
| 2 | AA, CA, DA, G, GA... (C, D, A, E, B...) | GLYCEROL | non-polymer | 92.1 | 23 | Chemie (GOL) | |||
| 3 | EA, FA, H, LA, Q... (D, A, E, B, F...) | SULFATE ION | non-polymer | 96.1 | 9 | Chemie (SO4) | |||
| 4 | HA, I, L, MA, R... (D, A, E, B, C) | CHLORIDE ION | non-polymer | 35.5 | 6 | Chemie (CL) | |||
| 5 | BA, IA, N, QA, S... (C, D, A, E, B...) | SODIUM ION | non-polymer | 23.0 | 6 | Chemie (NA) | |||
| 6 | AB, BB, CB, DB, YA... (C, D, E, F, A...) | water | water | 18.0 | 1273 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F: 1 - 271 (UniProt: P22862)
| PDB | External Database | Details |
|---|---|---|
| Ile 29 | Leu 30 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 6 |
| Total formula weight | 179963.7 | |
| Non-Polymers* | Number of molecules | 44 |
| Total formula weight | 3333.4 | |
| All* | Total formula weight | 183297.1 |
