3T4M

Ac-AChBP ligand binding domain mutated to human alpha-7 nAChR (intermediate)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C, D, E... (A, B, C, D, E...)	Soluble acetylcholine receptor	polymer	230	26347.1	10	UniProt (Q8WSF8) Pfam (PF02931)	Aplysia californica (California sea hare)
2	AA, EA, K, M, P... (H, J, A, B, C...)	CALCIUM ION	non-polymer		40.1	9	Chemie (CA)
3	BA, CA, DA, L, O... (H, I, A, B, D...)	(4R)-2-METHYLPENTANE-2,4-DIOL	non-polymer		118.2	9	Chemie (MRD)
4	N, Y (B, G)	(4S)-2-METHYL-2,4-PENTANEDIOL	non-polymer		118.2	2	Chemie (MPD)
5	V (F)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	1	Chemie (NAG)
6	FA, GA, HA, IA, JA... (A, B, C, D, E...)	water	water		18.0	91	Chemie (HOH)

Sequence modifications

A, B, C, D, E, F, G, H, I, J: 1 - 219 (UniProt: Q8WSF8)

PDB	External Database	Details
Asp -8	-	expression tag
Tyr -7	-	expression tag
Lys -6	-	expression tag
Asp -5	-	expression tag
Asp -4	-	expression tag
Asp -3	-	expression tag
Asp -2	-	expression tag
Lys -1	-	expression tag
Leu 0	-	expression tag
Trp 55	Tyr 72	engineered mutation
Asn 106	Ile 123	engineered mutation
Leu 108	Val 125	engineered mutation
Gln 116	Met 133	engineered mutation
Tyr 117	Phe 134	engineered mutation
Leu 118	Ile 135	engineered mutation
Ser 148	Val 165	engineered mutation
Arg 186	Gln 203	engineered mutation
Phe 187	His 204	engineered mutation
Glu 189	Ser 206	engineered mutation
Lys 192	Pro 209	engineered mutation
Pro 196	Ile 213	engineered mutation
Ser 220	-	expression tag
Arg 221	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	10
	Total formula weight	263471.4
Non-Polymers*	Number of molecules	21
	Total formula weight	1881.8
All*	Total formula weight	265353.2

*Water molecules are not included.