3T41

1.95 Angstrom Resolution Crystal Structure of Epidermin Leader Peptide Processing Serine Protease (EpiP) S393A Mutant from Staphylococcus aureus

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, B)	Epidermin leader peptide processing serine protease EpiP	polymer	471	52700.8	2	UniProt (Q5HEV5) Pfam (PF22386) Pfam (PF00082) UniProt (by SIFTS) (A0A0H2WX20)	Staphylococcus aureus
2	C, D, E, F, G... (A, B)	CALCIUM ION	non-polymer		40.1	6	Chemie (CA)
3	I (B)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
4	J, K (A, B)	water	water		18.0	487	Chemie (HOH)

Sequence modifications

A, B: 28 - 457 (UniProt: Q5HEV5)

PDB	External Database	Details
Met -13	-	expression tag
His -12	-	expression tag
His -11	-	expression tag
His -10	-	expression tag
His -9	-	expression tag
His -8	-	expression tag
His -7	-	expression tag
Ser -6	-	expression tag
Ser -5	-	expression tag
Gly -4	-	expression tag
Val -3	-	expression tag
Asp -2	-	expression tag
Leu -1	-	expression tag
Gly 0	-	expression tag
Thr 1	-	expression tag
Glu 2	-	expression tag
Asn 3	-	expression tag
Leu 4	-	expression tag
Tyr 5	-	expression tag
Phe 6	-	expression tag
Gln 7	-	expression tag
Ser 8	-	expression tag
Asn 9	-	expression tag
Ala 10	-	expression tag
Met 11	-	expression tag
Gly 12	-	expression tag
Ser 13	-	expression tag
Ser 14	-	expression tag
His 15	-	expression tag
His 16	-	expression tag
His 17	-	expression tag
Tyr 18	-	expression tag
His 19	-	expression tag
His 20	-	expression tag
Glu 21	-	expression tag
Asn 22	-	expression tag
Leu 23	-	expression tag
Tyr 24	-	expression tag
Phe 25	-	expression tag
Gln 26	-	expression tag
Gly 27	-	expression tag
Ala 393	Ser 393	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	105401.6
Non-Polymers*	Number of molecules	7
	Total formula weight	275.9
All*	Total formula weight	105677.5

*Water molecules are not included.