3T1Y

Structure of the Thermus thermophilus 30S ribosomal subunit complexed with a human anti-codon stem loop (HASL) of transfer RNA Lysine 3 (TRNALYS3) bound to an mRNA with an AAG-codon in the A-site and paromomycin

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	16S rRNA	polymer	1513	491166.8	1	GenBank (55771382)	Thermus thermophilus
2	B	30S ribosomal protein S2	polymer	256	29317.7	1	UniProt (P80371) Pfam (PF00318) In PDB	Thermus thermophilus
3	C	30S ribosomal protein S3	polymer	239	26751.1	1	UniProt (P80372) Pfam (PF07650) Pfam (PF00189) In PDB	Thermus thermophilus
4	D	30S ribosomal protein S4	polymer	209	24373.4	1	UniProt (P80373) Pfam (PF00163) Pfam (PF01479) In PDB	Thermus thermophilus
5	E	30S ribosomal protein S5	polymer	162	17583.4	1	UniProt (Q5SHQ5) Pfam (PF00333) Pfam (PF03719) In PDB	Thermus thermophilus
6	F	30S ribosomal protein S6	polymer	101	11988.8	1	UniProt (Q5SLP8) Pfam (PF01250) In PDB	Thermus thermophilus	TS9
7	G	30S ribosomal protein S7	polymer	156	18051.0	1	UniProt (P17291) Pfam (PF00177) In PDB	Thermus thermophilus
8	H	30S ribosomal protein S8	polymer	138	15868.6	1	UniProt (Q5SHQ2) Pfam (PF00410) UniProt (by SIFTS) (P0DOY9) In PDB	Thermus thermophilus
9	I	30S ribosomal protein S9	polymer	128	14429.7	1	UniProt (P62669) Pfam (PF00380) UniProt (by SIFTS) (P80374) In PDB	Thermus thermophilus
10	J	30S ribosomal protein S10	polymer	105	11955.0	1	UniProt (Q5SHN7) Pfam (PF00338) In PDB	Thermus thermophilus
11	K	30S ribosomal protein S11	polymer	129	13737.9	1	UniProt (P80376) Pfam (PF00411) In PDB	Thermus thermophilus
12	L	30S ribosomal protein S12	polymer	132	14637.4	1	UniProt (Q5SHN3) Pfam (PF00164) In PDB	Thermus thermophilus
13	M	30S ribosomal protein S13	polymer	126	14338.9	1	UniProt (P80377) Pfam (PF00416) In PDB	Thermus thermophilus
14	N	30S ribosomal protein S14 type Z	polymer	61	7158.7	1	UniProt (Q5SHQ1) Pfam (PF00253) UniProt (by SIFTS) (P0DOY6) In PDB	Thermus thermophilus
15	O	30S ribosomal protein S15	polymer	89	10578.4	1	UniProt (Q5SJ76) Pfam (PF00312) In PDB	Thermus thermophilus
16	P	30S ribosomal protein S16	polymer	88	10410.0	1	UniProt (Q5SJH3) Pfam (PF00886) In PDB	Thermus thermophilus
17	Q	30S ribosomal protein S17	polymer	105	12324.7	1	UniProt (P24321) Pfam (PF00366) UniProt (by SIFTS) (P0DOY7) In PDB	Thermus thermophilus
18	R	30S ribosomal protein S18	polymer	88	10244.3	1	UniProt (P80382) Pfam (PF01084) UniProt (by SIFTS) (Q5SLQ0) In PDB	Thermus thermophilus
19	S	30S ribosomal protein S19	polymer	93	10605.5	1	UniProt (Q5SHP2) Pfam (PF00203) In PDB	Thermus thermophilus
20	T	30S ribosomal protein S20	polymer	106	11722.1	1	UniProt (P62661) Pfam (PF01649) UniProt (by SIFTS) (P80380) In PDB	Thermus thermophilus
21	V	30S ribosomal protein Thx	polymer	27	3350.0	1	UniProt (Q5SIH3) Pfam (PF17070) In PDB	Thermus thermophilus	S31
22	W	mRNA A-site fragment	polymer	3	958.7	1
23	X	tRNA ASL human Lys3	polymer	11	3708.4	1
24	A, B	MAGNESIUM ION	non-polymer		24.3	186	Chemie (MG)
25	A	PAROMOMYCIN	non-polymer		615.6	1	Chemie (PAR)
26	D, N	ZINC ION	non-polymer		65.4	2	Chemie (ZN)

Sequence modifications

A: 5 - 1516 (GenBank: 55771382)

PDB	External Database	Details
U 1517	-	insertion
U 1518	-	insertion
U 1519	-	insertion
C 1520	-	insertion
U 1521	-	insertion

Contents of the asymmetric unit

Polymers	Number of chains	23
	Total formula weight	785260.2
Non-Polymers*	Number of molecules	189
	Total formula weight	5267.2
All*	Total formula weight	790527.4

*Water molecules are not included.