3T1G
Engineering of organophosphate hydrolase by computational design and directed evolution
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | organophosphate hydrolase | polymer | 353 | 40180.7 | 1 | UniProt (P03958) Pfam (PF00962) | Mus musculus (mouse) | |
| 2 | B, C, D, E (A) | ZINC ION | non-polymer | 65.4 | 4 | Chemie (ZN) | |||
| 3 | F (A) | CALCIUM ION | non-polymer | 40.1 | 1 | Chemie (CA) | |||
| 4 | G (A) | water | water | 18.0 | 136 | Chemie (HOH) |
Sequence modifications
A: 4 - 352 (UniProt: P03958)
| PDB | External Database | Details |
|---|---|---|
| Gly 0 | - | expression tag |
| Ser 1 | - | expression tag |
| His 2 | - | expression tag |
| Met 3 | - | expression tag |
| Ser 19 | Asp 19 | engineered mutation |
| Gln 58 | Leu 58 | engineered mutation |
| Thr 61 | Phe 61 | engineered mutation |
| Trp 65 | Phe 65 | engineered mutation |
| His 138 | Gln 138 | engineered mutation |
| Ile 183 | Ala 183 | engineered mutation |
| Phe 218 | Val 218 | engineered mutation |
| Ala 296 | Asp 296 | engineered mutation |
| Glu 299 | Ile 299 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 40180.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 301.7 | |
| All* | Total formula weight | 40482.4 |
