3SZG
Crystal structure of C176A glutamine-dependent NAD+ synthetase from M. tuberculosis bound to AMP/PPi and NaAD+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D (A, B, C, D) | Glutamine-dependent NAD(+) synthetase | polymer | 680 | 74823.4 | 4 | UniProt (P0A5L6) Pfam (PF00795) Pfam (PF02540) UniProt (by SIFTS) (P9WJJ3) | Mycobacterium tuberculosis | NAD(+) synthase [glutamine-hydrolyzing] |
| 2 | E, H, L, P (A, B, C, D) | NICOTINIC ACID ADENINE DINUCLEOTIDE | non-polymer | 665.4 | 4 | Chemie (DND) | |||
| 3 | F, I, M, Q (A, B, C, D) | ADENOSINE MONOPHOSPHATE | non-polymer | 347.2 | 4 | Chemie (AMP) PubChem (16397049) PubChem (16760266) PubChem (40459109) PubChem (46875232) PubChem (40467049) PubChem (40469468) PubChem (44348067) PubChem (45358029) PubChem (177678969) PubChem (6083) PubChem (46874558) PubChem (1549371) PubChem (92144436) PubChem (57358045) PubChem (12358354) PubChem (57879893) PubChem (57879902) PubChem (224) PubChem (122197642) PubChem (34768) PubChem (83862) PubChem (446048) PubChem (515384) PubChem (638042) PubChem (5316444) PubChem (5742864) PubChem (12163610) PubChem (12358355) PubChem (162290139) PubChem (162946266) PubChem (171699505) | |||
| 4 | G, J, N, R (A, B, C, D) | PYROPHOSPHATE 2- | non-polymer | 176.0 | 4 | Chemie (POP) PubChem (4995) | |||
| 5 | K, O, S (B, C, D) | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) PubChem (753) | |||
| 6 | T, U, V, W (A, B, C, D) | water | water | 18.0 | 745 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 679 (UniProt: P0A5L6)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 176 | Cys 176 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 299293.4 | |
| Non-Polymers* | Number of molecules | 15 |
| Total formula weight | 5030.7 | |
| All* | Total formula weight | 304324.1 |
