3ST0

Engineered medium-affinity halide-binding protein derived from YFP: halide-free

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Green fluorescent protein	polymer	260	29307.7	1	UniProt (P42212) Pfam (PF01353) In PDB	Aequorea victoria (Jellyfish)
2	A	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
3	A	FORMIC ACID	non-polymer		46.0	1	Chemie (FMT)
4		water	water		18.0	400	Chemie (HOH)

Sequence modifications

A: 0 - 238 (UniProt: P42212)

PDB	External Database	Details
Val 1	-	insertion
Cr2 66	Ser 65	chromophore
Cr2 66	Tyr 66	chromophore
Cr2 66	Gly 67	chromophore
Thr 69	Gln 69	engineered mutation
Ala 72	Ser 72	engineered mutation
Arg 79	Lys 79	engineered mutation
Ala 163	Val 163	engineered mutation
Tyr 203	Thr 203	engineered mutation
Leu 231	His 231	engineered mutation
Gly 239	-	expression tag
Gly 240	-	expression tag
Ser 241	-	expression tag
Asn 242	-	expression tag
Asp 243	-	expression tag
Tyr 244	-	expression tag
Lys 245	-	expression tag
Asp 246	-	expression tag
Asp 247	-	expression tag
Asp 248	-	expression tag
Asp 249	-	expression tag
Lys 250	-	expression tag
Gly 251	-	expression tag
Gly 252	-	expression tag
Ser 253	-	expression tag
His 254	-	expression tag
His 255	-	expression tag
His 256	-	expression tag
His 257	-	expression tag
His 258	-	expression tag
His 259	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	29307.7
Non-Polymers*	Number of molecules	2
	Total formula weight	108.1
All*	Total formula weight	29415.8

*Water molecules are not included.