3SPZ
DNA Polymerase(L415A/L561A/S565G/Y567A) Ternary Complex with dUpCpp Opposite dA (Ca2+)
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | DNA polymerase | polymer | 903 | 104448.9 | 1 | UniProt (Q38087) Pfam (PF03104) Pfam (PF00136) | Enterobacteria phage RB69 | Gp43 |
| 2 | B (T) | 5'-D(P*GP*AP*GP*TP*AP*AP*GP*CP*AP*GP*TP*CP*CP*GP*CP*G)-3' | polymer | 16 | 4948.2 | 1 | |||
| 3 | C (P) | 5'-D(*GP*CP*GP*GP*AP*CP*TP*GP*CP*TP*TP*AP*C)-3' | polymer | 13 | 3967.6 | 1 | |||
| 4 | D (A) | 2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]uridine | non-polymer | 466.2 | 1 | Chemie (UPC) | |||
| 5 | E, F, G, H (A) | CALCIUM ION | non-polymer | 40.1 | 4 | Chemie (CA) | |||
| 6 | I, J, K (A, T, P) | water | water | 18.0 | 229 | Chemie (HOH) |
Sequence modifications
A: 1 - 903 (UniProt: Q38087)
| PDB | External Database | Details |
|---|---|---|
| Ala 222 | Asp 222 | engineered mutation |
| Ala 327 | Asp 327 | engineered mutation |
| Ala 415 | Leu 415 | engineered mutation |
| Ala 561 | Leu 561 | engineered mutation |
| Gly 565 | Ser 565 | engineered mutation |
| Ala 567 | Tyr 567 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 113364.7 | |
| Non-Polymers* | Number of molecules | 5 |
| Total formula weight | 626.5 | |
| All* | Total formula weight | 113991.2 |
