3SK0

structure of Rhodococcus rhodochrous haloalkane dehalogenase DhaA mutant DhaA12

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Haloalkane dehalogenase	polymer	311	35397.0	1	UniProt (P0A3G2) Pfam (PF00561)	Rhodococcus rhodochrous
2	B (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
3	C (A)	water	water		18.0	270	Chemie (HOH)

Sequence modifications

A: 1 - 304 (UniProt: P0A3G2)

PDB	External Database	Details
Phe 141	Trp 141	engineered mutation
His 142	-	insertion
His 143	-	insertion
Thr 144	-	insertion
Glu 145	-	insertion
Val 146	-	insertion
Ala 147	-	insertion
Glu 148	-	insertion
Glu 149	-	insertion
Gln 150	-	insertion
Asp 151	-	insertion
His 152	-	insertion
Ala 153	Pro 142	engineered mutation
Ala 155	Phe 144	engineered mutation
Arg 182	Gly 171	engineered mutation
Val 183	Ala 172	engineered mutation
Gly 186	Lys 175	engineered mutation
Gly 187	Cys 176	engineered mutation
Ala 256	Val 245	engineered mutation
Glu 305	-	expression tag
His 306	-	expression tag
His 307	-	expression tag
His 308	-	expression tag
His 309	-	expression tag
His 310	-	expression tag
His 311	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	35397.0
Non-Polymers*	Number of molecules	1
	Total formula weight	35.5
All*	Total formula weight	35432.5

*Water molecules are not included.