3SJX

X-ray structure of human glutamate carboxypeptidase II (the E424A inactive mutant) in complex with N-acetyl-aspartyl-methionine

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Glutamate carboxypeptidase 2	polymer	709	79801.0	1	UniProt (Q04609) Pfam (PF02225) Pfam (PF04389) Pfam (PF04253)	Homo sapiens (human)	Cell growth-inhibiting gene 27 protein, Folate hydrolase 1, Folylpoly-gamma-glutamate carboxypeptidase, FGCP, Glutamate carboxypeptidase II, GCPII, Membrane glutamate carboxypeptidase, mGCP, N-acetylated-alpha-linked acidic dipeptidase I, NAALADase I, Prostate-specific membrane antigen, PSM, PSMA, Pteroylpoly-gamma-glutamate carboxypeptidase
2	B, C (B, C)	2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		424.4	2	In PDB GlyTouCan (G42666HT)
3	D (D)	alpha-D-mannopyranose-(1-3)-beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose	branched		748.7	1	In PDB GlyTouCan (G81315DD)
4	E, F (A)	ZINC ION	non-polymer		65.4	2	Chemie (ZN)
5	G (A)	CALCIUM ION	non-polymer		40.1	1	Chemie (CA)
6	H (A)	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
7	I, J, K (A)	2-acetamido-2-deoxy-beta-D-glucopyranose	non-polymer		221.2	3	Chemie (NAG)
8	L (A)	N-acetyl-L-alpha-aspartyl-L-methionine	non-polymer		306.3	1	BIRD (PRD_000799) Chemie (QRG)
9	M (A)	water	water		18.0	457	Chemie (HOH)

Sequence modifications

A: 44 - 750 (UniProt: Q04609)

PDB	External Database	Details
Arg 42	-	expression tag
Ser 43	-	expression tag
Ala 424	Glu 424	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	79801.0
Branched	Number of molecules	3
	Total formula weight	1597.5
Non-Polymers*	Number of molecules	8
	Total formula weight	1176.3
All*	Total formula weight	82574.8

*Water molecules are not included.