3SH1
Ac-AChBP ligand binding domain mutated to human alpha-7 nAChR
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D, E... (A, B, C, D, E...) | Soluble acetylcholine receptor | polymer | 230 | 26323.1 | 10 | UniProt (Q8WSF8) Pfam (PF02931) | Aplysia californica (California sea hare) | |
| 2 | K, L, M, N (K, L, M, N) | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 424.4 | 4 | In PDB GlyTouCan (G42666HT) | |||
| 3 | O, P (O, P) | beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose | branched | 586.5 | 2 | In PDB GlyTouCan (G15407YE) | |||
| 4 | AA, GA, Q, SA, V (C, E, A, I, B) | MAGNESIUM ION | non-polymer | 24.3 | 5 | Chemie (MG) | |||
| 5 | CA, FA, JA, MA, OA... (C, D, E, F, G...) | METHYLLYCACONITINE | non-polymer | 682.8 | 10 | Chemie (MLK) | |||
| 6 | EA, HA, KA, S, Z (D, E, F, A, B) | (4R)-2-METHYLPENTANE-2,4-DIOL | non-polymer | 118.2 | 5 | Chemie (MRD) | |||
| 7 | DA, PA, QA, T, U... (C, G, A, I, J) | (4S)-2-METHYL-2,4-PENTANEDIOL | non-polymer | 118.2 | 7 | Chemie (MPD) | |||
| 8 | BA, IA, LA, TA, UA... (C, E, F, I, B) | 2-acetamido-2-deoxy-beta-D-glucopyranose | non-polymer | 221.2 | 7 | Chemie (NAG) | |||
| 9 | NA (G) | ACETATE ION | non-polymer | 59.0 | 1 | Chemie (ACT) | |||
| 10 | AB, BB, CB, DB, EB... (B, C, D, E, F...) | water | water | 18.0 | 71 | Chemie (HOH) |
Sequence modifications
A, B, C, D, E, F, G, H, I, J: 1 - 219 (UniProt: Q8WSF8)
| PDB | External Database | Details |
|---|---|---|
| Asp -8 | - | expression tag |
| Tyr -7 | - | expression tag |
| Lys -6 | - | expression tag |
| Asp -5 | - | expression tag |
| Asp -4 | - | expression tag |
| Asp -3 | - | expression tag |
| Asp -2 | - | expression tag |
| Lys -1 | - | expression tag |
| Leu 0 | - | expression tag |
| Tyr 32 | Thr 49 | engineered mutation |
| Ser 34 | Gly 51 | engineered mutation |
| Ser 36 | Thr 53 | engineered mutation |
| Leu 38 | Gln 55 | engineered mutation |
| Trp 55 | Tyr 72 | engineered mutation |
| Ser 59 | Arg 76 | engineered mutation |
| Asn 106 | Ile 123 | engineered mutation |
| Leu 108 | Val 125 | engineered mutation |
| Asn 110 | Thr 127 | engineered mutation |
| Ser 111 | His 128 | engineered mutation |
| Ser 112 | Asp 129 | engineered mutation |
| His 114 | Ser 131 | engineered mutation |
| Gln 116 | Met 133 | engineered mutation |
| Tyr 117 | Phe 134 | engineered mutation |
| Leu 118 | Ile 135 | engineered mutation |
| Ser 148 | Val 165 | engineered mutation |
| Gly 150 | Ser 167 | engineered mutation |
| Trp 152 | Phe 169 | engineered mutation |
| Ser 184 | Gln 201 | engineered mutation |
| Glu 185 | Val 202 | engineered mutation |
| Arg 186 | Gln 203 | engineered mutation |
| Phe 187 | His 204 | engineered mutation |
| Glu 189 | Ser 206 | engineered mutation |
| Lys 192 | Pro 209 | engineered mutation |
| Pro 196 | Ile 213 | engineered mutation |
| Ser 220 | - | expression tag |
| Arg 221 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 10 |
| Total formula weight | 263230.5 | |
| Branched | Number of molecules | 6 |
| Total formula weight | 2870.7 | |
| Non-Polymers* | Number of molecules | 35 |
| Total formula weight | 9975.1 | |
| All* | Total formula weight | 276076.3 |
