3SG2

Crystal Structure of GCaMP2-T116V,D381Y

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Myosin light chain kinase, Green fluorescent protein, Calmodulin-1 chimera	polymer	451	50750.7	1	UniProt (Q6LDG3) UniProt (P42212) UniProt (P0DP29) Pfam (PF01353) Pfam (PF13499) UniProt (by SIFTS) (P11799) In PDB	Gallus gallus (Chicken)
2	A	CALCIUM ION	non-polymer		40.1	4	Chemie (CA)
3		water	water		18.0	278	Chemie (HOH)

Sequence modifications

A: 41 - 59 (UniProt: Q6LDG3)

PDB	External Database	Details
Met 1	-	expression tag
Arg 2	-	expression tag
Gly 3	-	expression tag
Ser 4	-	expression tag
His 5	-	expression tag
His 6	-	expression tag
His 7	-	expression tag
His 8	-	expression tag
His 9	-	expression tag
His 10	-	expression tag
Gly 11	-	expression tag
Met 12	-	expression tag
Ala 13	-	expression tag
Ser 14	-	expression tag
Met 15	-	expression tag
Thr 16	-	expression tag
Gly 17	-	expression tag
Gly 18	-	expression tag
Gln 19	-	expression tag
Gln 20	-	expression tag
Met 21	-	expression tag
Gly 22	-	expression tag
Arg 23	-	expression tag
Asp 24	-	expression tag
Leu 25	-	expression tag
Tyr 26	-	expression tag
Asp 27	-	expression tag
Asp 28	-	expression tag
Asp 29	-	expression tag
Asp 30	-	expression tag
Lys 31	-	expression tag
Asp 32	-	expression tag
Leu 33	-	expression tag
Ala 34	-	expression tag
Thr 35	-	expression tag
Met 36	-	expression tag
Val 37	-	expression tag
Asp 38	-	expression tag
Ser 39	-	expression tag
Ser 40	-	expression tag
Asn 45	Gln 41	engineered mutation
Leu 60	-	linker
Glu 61	-	linker

A: 62 - 151 (UniProt: P42212)

PDB	External Database	Details
Ala 76	Val 163	engineered mutation
Gly 88	Ser 175	engineered mutation
Tyr 93	Asp 180	engineered mutation
Val 116	Thr 203	engineered mutation
Lys 119	Ala 206	engineered mutation
Leu 144	His 231	engineered mutation

A: 160 - 302 (UniProt: P42212)

PDB	External Database	Details
Gly 152	-	linker
Gly 153	-	linker
Thr 154	-	linker
Gly 155	-	linker
Gly 156	-	linker
Ser 157	-	linker
Met 158	-	linker
Val 159	-	linker
Leu 222	Phe 64	engineered mutation
-	Ser 65	deletion
-	Tyr 66	deletion
Cro 224	Gly 67	chromophore
Ile 251	Val 93	engineered mutation
Thr 303	-	linker
Arg 304	-	linker

A: 305 - 451 (UniProt: P0DP29)

PDB	External Database	Details
Tyr 381	Asp 79	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	50750.7
Non-Polymers*	Number of molecules	4
	Total formula weight	160.3
All*	Total formula weight	50911.0

*Water molecules are not included.