3SEY
Zn-mediated Polymer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form II)
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, C, E | Maltose-binding periplasmic protein | polymer | 372 | 40698.0 | 3 | UniProt (P0AEX9) Pfam (PF01547) In PDB | Escherichia coli | MMBP, Maltodextrin-binding protein |
2 | B, D, F | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 3 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
3 | C, A, E | ZINC ION | non-polymer | 65.4 | 23 | Chemie (ZN) | |||
4 | C, E, A | ACETATE ION | non-polymer | 59.0 | 8 | Chemie (ACT) | |||
5 | C, A | GLYCEROL | non-polymer | 92.1 | 5 | Chemie (GOL) | |||
6 | water | water | 18.0 | 480 | Chemie (HOH) |
Sequence modifications
A, C, E: 2 - 359 (UniProt: P0AEX9)
PDB | External Database | Details |
---|---|---|
Met 1 | - | initiating methionine |
His 216 | Ala 241 | engineered mutation |
His 220 | Lys 245 | engineered mutation |
Ala 360 | - | expression tag |
Ala 361 | - | expression tag |
Leu 362 | - | expression tag |
Ala 363 | - | expression tag |
Ala 364 | - | expression tag |
Ala 365 | - | expression tag |
Gln 366 | - | expression tag |
Thr 367 | - | expression tag |
Asn 368 | - | expression tag |
Ala 369 | - | expression tag |
Ala 370 | - | expression tag |
Ala 371 | - | expression tag |
Ala 372 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 122094.1 | |
Branched | Number of molecules | 3 |
Total formula weight | 1026.9 | |
Non-Polymers* | Number of molecules | 36 |
Total formula weight | 2437.2 | |
All* | Total formula weight | 125558.2 |