3SEW

Zn-mediated Polymer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A (A)	Maltose-binding periplasmic protein	polymer	372	40698.0	1	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli	MMBP, Maltodextrin-binding protein
2	B (B)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	1	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	C (A)	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	D, E, F, J (A)	CHLORIDE ION	non-polymer		35.5	4	Chemie (CL)
5	G, H, I, K, L (A)	GLYCEROL	non-polymer		92.1	5	Chemie (GOL)
6	M (A)	water	water		18.0	268	Chemie (HOH)

Sequence modifications

A: 2 - 359 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
His 216	Ala 241	engineered mutation
His 220	Lys 245	engineered mutation
Ala 360	-	expression tag
Ala 361	-	expression tag
Leu 362	-	expression tag
Ala 363	-	expression tag
Ala 364	-	expression tag
Ala 365	-	expression tag
Gln 366	-	expression tag
Thr 367	-	expression tag
Asn 368	-	expression tag
Ala 369	-	expression tag
Ala 370	-	expression tag
Ala 371	-	expression tag
Ala 372	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40698.0
Branched	Number of molecules	1
	Total formula weight	342.3
Non-Polymers*	Number of molecules	10
	Total formula weight	667.7
All*	Total formula weight	41708.0

*Water molecules are not included.