3SEV

Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, C, E	Maltose-binding periplasmic protein	polymer	372	40640.0	3	UniProt (P0AEX9) Pfam (PF01547) In PDB	Escherichia coli	MMBP, Maltodextrin-binding protein
2	B, D, F	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	3	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	A, C, E	ZINC ION	non-polymer		65.4	3	Chemie (ZN)
4	A	CHLORIDE ION	non-polymer		35.5	1	Chemie (CL)
5		water	water		18.0	6	Chemie (HOH)

Sequence modifications

A, C, E: 2 - 359 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
His 310	Glu 335	engineered mutation
His 314	Lys 339	engineered mutation
Ala 360	-	expression tag
Ala 361	-	expression tag
Leu 362	-	expression tag
Ala 363	-	expression tag
Ala 364	-	expression tag
Ala 365	-	expression tag
Gln 366	-	expression tag
Thr 367	-	expression tag
Asn 368	-	expression tag
Ala 369	-	expression tag
Ala 370	-	expression tag
Ala 371	-	expression tag
Ala 372	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	121919.9
Branched	Number of molecules	3
	Total formula weight	1026.9
Non-Polymers*	Number of molecules	4
	Total formula weight	231.7
All*	Total formula weight	123178.5

*Water molecules are not included.