3SEV
Zn-mediated Trimer of Maltose-binding Protein E310H/K314H by Synthetic Symmetrization
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C (A, C, E) | Maltose-binding periplasmic protein | polymer | 372 | 40640.0 | 3 | UniProt (P0AEX9) Pfam (PF01547) | Escherichia coli | MMBP, Maltodextrin-binding protein |
| 2 | D, E, F (B, D, F) | alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose | branched | 342.3 | 3 | In PDB BIRD (PRD_900001) GlyTouCan (G07411ON) | alpha-maltose | ||
| 3 | G, I, J (A, C, E) | ZINC ION | non-polymer | 65.4 | 3 | Chemie (ZN) | |||
| 4 | H (A) | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
| 5 | K, L (A, C) | water | water | 18.0 | 6 | Chemie (HOH) |
Sequence modifications
A, C, E: 2 - 359 (UniProt: P0AEX9)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | initiating methionine |
| His 310 | Glu 335 | engineered mutation |
| His 314 | Lys 339 | engineered mutation |
| Ala 360 | - | expression tag |
| Ala 361 | - | expression tag |
| Leu 362 | - | expression tag |
| Ala 363 | - | expression tag |
| Ala 364 | - | expression tag |
| Ala 365 | - | expression tag |
| Gln 366 | - | expression tag |
| Thr 367 | - | expression tag |
| Asn 368 | - | expression tag |
| Ala 369 | - | expression tag |
| Ala 370 | - | expression tag |
| Ala 371 | - | expression tag |
| Ala 372 | - | expression tag |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 3 |
| Total formula weight | 121919.9 | |
| Branched | Number of molecules | 3 |
| Total formula weight | 1026.9 | |
| Non-Polymers* | Number of molecules | 4 |
| Total formula weight | 231.7 | |
| All* | Total formula weight | 123178.5 |
