3SET

Ni-mediated Dimer of Maltose-binding Protein A216H/K220H by Synthetic Symmetrization (Form I)

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B (A, C)	Maltose-binding periplasmic protein	polymer	372	40698.0	2	UniProt (P0AEX9) Pfam (PF01547)	Escherichia coli	MMBP, Maltodextrin-binding protein
2	C, D (B, D)	alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose	branched		342.3	2	In PDB BIRD (PRD_900001) GlyTouCan (G07411ON)		alpha-maltose
3	E, F (A, C)	NICKEL (II) ION	non-polymer		58.7	2	Chemie (NI)
4	G, H (A, C)	water	water		18.0	286	Chemie (HOH)

Sequence modifications

A, C: 2 - 359 (UniProt: P0AEX9)

PDB	External Database	Details
Met 1	-	initiating methionine
His 216	Ala 241	engineered mutation
His 220	Lys 245	engineered mutation
Ala 360	-	expression tag
Ala 361	-	expression tag
Leu 362	-	expression tag
Ala 363	-	expression tag
Ala 364	-	expression tag
Ala 365	-	expression tag
Gln 366	-	expression tag
Thr 367	-	expression tag
Asn 368	-	expression tag
Ala 369	-	expression tag
Ala 370	-	expression tag
Ala 371	-	expression tag
Ala 372	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	81396.0
Branched	Number of molecules	2
	Total formula weight	684.6
Non-Polymers*	Number of molecules	2
	Total formula weight	117.4
All*	Total formula weight	82198.0

*Water molecules are not included.