3SEQ
Crystal structure of C176A mutant of glutamine-dependent NAD+ synthetase from M. tuberculosis in complex with AMPCPP and NaAD+
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B, C, D | Glutamine-dependent NAD(+) synthetase | polymer | 680 | 74823.4 | 4 | UniProt (P0A5L6) Pfam (PF00795) Pfam (PF02540) UniProt (by SIFTS) (P9WJJ3) In PDB | Mycobacterium tuberculosis | NAD(+) synthase [glutamine-hydrolyzing] |
| 2 | A, B, C, D | DIPHOSPHOMETHYLPHOSPHONIC ACID ADENOSYL ESTER | non-polymer | 505.2 | 4 | Chemie (APC) | |||
| 3 | A, B, C, D | NICOTINIC ACID ADENINE DINUCLEOTIDE | non-polymer | 665.4 | 4 | Chemie (DND) | |||
| 4 | B, D | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 5 | water | water | 18.0 | 349 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 1 - 679 (UniProt: P0A5L6)
| PDB | External Database | Details |
|---|---|---|
| Ser 0 | - | expression tag |
| Ala 176 | Cys 176 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 4 |
| Total formula weight | 299293.4 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 4866.7 | |
| All* | Total formula weight | 304160.1 |
