3SCU

Crystal Structure of Rice BGlu1 E386G Mutant Complexed with Cellopentaose

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Beta-glucosidase 7	polymer	481	54656.4	2	UniProt (Q75I93) Pfam (PF00232) In PDB	Oryza sativa Japonica Group (Japanese rice)	Os3bglu7
2	C, D	beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-4)-beta-D-glucopyranose	branched		828.7	2	In PDB BIRD (PRD_900016) GlyTouCan (G91683DU)		beta-cellopentaose
3	A	ZINC ION	non-polymer		65.4	1	Chemie (ZN)
4	A, B	SULFATE ION	non-polymer		96.1	2	Chemie (SO4)
5	A, B	2-(N-MORPHOLINO)-ETHANESULFONIC ACID	non-polymer		195.2	2	Chemie (MES)
6		water	water		18.0	922	Chemie (HOH)

Sequence modifications

A, B: 1 - 476 (UniProt: Q75I93)

PDB	External Database	Details
Ala -4	-	expression tag
Met -3	-	expression tag
Ala -2	-	expression tag
Asp -1	-	expression tag
Val 0	-	expression tag
Val 24	Ala 52	SEE REMARK 999
Gly 386	Glu 414	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	109312.8
Branched	Number of molecules	2
	Total formula weight	1657.4
Non-Polymers*	Number of molecules	5
	Total formula weight	648.0
All*	Total formula weight	111618.3

*Water molecules are not included.