3SCS
Crystal Structure of Rice BGlu1 E386S Mutant Complexed with alpha-Glucosyl Fluoride
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-glucosidase 7 | polymer | 481 | 54686.4 | 2 | UniProt (Q75I93) Pfam (PF00232) | Oryza sativa Japonica Group (Japanese rice) | Os3bglu7 |
| 2 | C (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | D, H (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E, I (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) PubChem (4478249) | |||
| 5 | F, J (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) PubChem (753) | |||
| 6 | G, K (A, B) | alpha-D-glucopyranosyl fluoride | non-polymer | 182.1 | 2 | Chemie (GLF) PubChem (21600205) PubChem (150967) PubChem (444915) PubChem (4465565) PubChem (10921145) PubChem (11126887) PubChem (11286835) PubChem (11412739) PubChem (11816235) PubChem (13643247) PubChem (129668793) PubChem (129685662) PubChem (131107081) PubChem (131215288) PubChem (133620782) PubChem (135085714) PubChem (157225707) PubChem (157225708) PubChem (163321034) PubChem (166604477) PubChem (172641965) | |||
| 7 | L, M (A, B) | water | water | 18.0 | 870 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 476 (UniProt: Q75I93)
| PDB | External Database | Details |
|---|---|---|
| Ala -4 | - | expression tag |
| Met -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Val 0 | - | expression tag |
| Val 24 | Ala 52 | SEE REMARK 999 |
| Ser 386 | Glu 414 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 109372.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1196.5 | |
| All* | Total formula weight | 110569.4 |
