3SCS
Crystal Structure of Rice BGlu1 E386S Mutant Complexed with alpha-Glucosyl Fluoride
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | Beta-glucosidase 7 | polymer | 481 | 54686.4 | 2 | UniProt (Q75I93) Pfam (PF00232) | Oryza sativa Japonica Group (Japanese rice) | Os3bglu7 |
| 2 | C (A) | ZINC ION | non-polymer | 65.4 | 1 | Chemie (ZN) | |||
| 3 | D, H (A, B) | SULFATE ION | non-polymer | 96.1 | 2 | Chemie (SO4) | |||
| 4 | E, I (A, B) | 2-(N-MORPHOLINO)-ETHANESULFONIC ACID | non-polymer | 195.2 | 2 | Chemie (MES) | |||
| 5 | F, J (A, B) | GLYCEROL | non-polymer | 92.1 | 2 | Chemie (GOL) | |||
| 6 | G, K (A, B) | alpha-D-glucopyranosyl fluoride | non-polymer | 182.1 | 2 | Chemie (GLF) | |||
| 7 | L, M (A, B) | water | water | 18.0 | 870 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 476 (UniProt: Q75I93)
| PDB | External Database | Details |
|---|---|---|
| Ala -4 | - | expression tag |
| Met -3 | - | expression tag |
| Ala -2 | - | expression tag |
| Asp -1 | - | expression tag |
| Val 0 | - | expression tag |
| Val 24 | Ala 52 | SEE REMARK 999 |
| Ser 386 | Glu 414 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 109372.9 | |
| Non-Polymers* | Number of molecules | 9 |
| Total formula weight | 1196.5 | |
| All* | Total formula weight | 110569.4 |
