3SB8

Cu-mediated Dimer of T4 Lysozyme D61H/K65H by Synthetic Symmetrization

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B, C (A, B, C)	Lysozyme	polymer	165	18644.3	3	UniProt (P00720) Pfam (PF00959)	Enterobacteria phage T4	Endolysin, Lysis protein, Muramidase
2	D, E (A, B)	COPPER (II) ION	non-polymer		63.5	2	Chemie (CU)
3	F, G, H (A, B, C)	water	water		18.0	12	Chemie (HOH)

Sequence modifications

A, B, C: 1 - 162 (UniProt: P00720)

PDB	External Database	Details
Gly -2	-	expression tag
Gly -1	-	expression tag
Pro 0	-	expression tag
Thr 54	Cys 54	engineered mutation
His 61	Asp 61	engineered mutation
His 65	Lys 65	engineered mutation
Ala 97	Cys 97	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	3
	Total formula weight	55933.0
Non-Polymers*	Number of molecules	2
	Total formula weight	127.1
All*	Total formula weight	56060.1

*Water molecules are not included.