3S4X

Crystal structure of the Asn152Gly mutant of P99 beta-lactamase

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	Beta-lactamase	polymer	367	40081.6	1	UniProt (P05364) Pfam (PF00144) In PDB	Enterobacter cloacae	Cephalosporinase
2	A	SULFATE ION	non-polymer		96.1	7	Chemie (SO4)
3		water	water		18.0	221	Chemie (HOH)

Sequence modifications

A: 1 - 361 (UniProt: P05364)

PDB	External Database	Details
Val 16	Ile 36	engineered mutation
Pro 88	Ala 108	engineered mutation
Gly 152	Asn 172	engineered mutation
Val 299	Ala 319	engineered mutation
His 362	-	expression tag
His 363	-	expression tag
His 364	-	expression tag
His 365	-	expression tag
His 366	-	expression tag
His 367	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	1
	Total formula weight	40081.6
Non-Polymers*	Number of molecules	7
	Total formula weight	672.4
All*	Total formula weight	40754.1

*Water molecules are not included.