3RV4
Crystal structure of E.coli biotin carboxylase R16E mutant in complex with Mg-ADP and bicarbonate
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A | Biotin carboxylase | polymer | 452 | 49640.8 | 1 | UniProt (P24182) Pfam (PF00289) Pfam (PF02786) Pfam (PF02785) In PDB | Escherichia coli | Acetyl-CoA carboxylase subunit A, ACC |
| 2 | A | ADENOSINE-5'-DIPHOSPHATE | non-polymer | 427.2 | 1 | Chemie (ADP) | |||
| 3 | A | MAGNESIUM ION | non-polymer | 24.3 | 1 | Chemie (MG) | |||
| 4 | A | BICARBONATE ION | non-polymer | 61.0 | 1 | Chemie (BCT) | |||
| 5 | A | CESIUM ION | non-polymer | 132.9 | 1 | Chemie (CS) | |||
| 6 | A | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 7 | A | SODIUM ION | non-polymer | 23.0 | 2 | Chemie (NA) | |||
| 8 | A | GLYCEROL | non-polymer | 92.1 | 1 | Chemie (GOL) | |||
| 9 | A | METHANOL | non-polymer | 32.0 | 1 | Chemie (MOH) | |||
| 10 | water | water | 18.0 | 365 | Chemie (HOH) |
Sequence modifications
A: 1 - 449 (UniProt: P24182)
| PDB | External Database | Details |
|---|---|---|
| Gly 1 | - | expression tag |
| Ser 1 | - | expression tag |
| His 1 | - | expression tag |
| Glu 16 | Arg 16 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 49640.8 | |
| Non-Polymers* | Number of molecules | 10 |
| Total formula weight | 886.5 | |
| All* | Total formula weight | 50527.3 |
