3RRN

S. cerevisiae dbp5 l327v bound to gle1 h337r and ip6

Replaces:  3PEX

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A	ATP-dependent RNA helicase DBP5	polymer	395	44172.0	1	UniProt (P20449) Pfam (PF00270) Pfam (PF00271) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	DEAD box protein 5, Helicase CA5/6, Ribonucleic acid-trafficking protein 8
2	B	Nucleoporin GLE1	polymer	297	34274.7	1	UniProt (Q12315) Pfam (PF07817) In PDB	Saccharomyces cerevisiae (brewer's yeast lager beer yeast yeast)	Nuclear pore protein GLE1, RNA export factor GLE1
3	A	ADENOSINE-5'-DIPHOSPHATE	non-polymer		427.2	1	Chemie (ADP)
4	A, B	INOSITOL HEXAKISPHOSPHATE	non-polymer		660.0	3	Chemie (IHP)

Sequence modifications

A: 91 - 482 (UniProt: P20449)

PDB	External Database	Details
Gly 88	-	expression tag
Ala 89	-	expression tag
Met 90	-	expression tag
Val 327	Leu 327	engineered mutation

B: 244 - 538 (UniProt: Q12315)

PDB	External Database	Details
Gly 242	-	expression tag
Ala 243	-	expression tag
Arg 337	His 337	engineered mutation

Contents of the asymmetric unit

Polymers	Number of chains	2
	Total formula weight	78446.7
Non-Polymers*	Number of molecules	4
	Total formula weight	2407.3
All*	Total formula weight	80854.0

*Water molecules are not included.