3RN1

Crystal Structure of the W199E-MauG/pre-Methylamine Dehydrogenase Complex

Entity

Entity ID	Chain ID	Description	Type	Chain length	Formula weight	Number of molecules	DB Name (Accession)	Biological source	Descriptive keywords
1	A, B	Methylamine utilization protein MauG	polymer	373	41089.5	2	UniProt (Q51658) Pfam (PF03150) In PDB	Paracoccus denitrificans
2	C, E	Methylamine dehydrogenase light chain	polymer	137	15025.6	2	UniProt (A1BBA0) Pfam (PF02975) In PDB	Paracoccus denitrificans
3	D, F	Methylamine dehydrogenase heavy chain	polymer	386	42449.3	2	UniProt (A1BB97) Pfam (PF06433) In PDB	Paracoccus denitrificans
4	A, B	CALCIUM ION	non-polymer		40.1	2	Chemie (CA)
5	A, B	SODIUM ION	non-polymer		23.0	4	Chemie (NA)
6	A, B	HEME C	non-polymer		618.5	4	Chemie (HEC)
7	B	1,2-ETHANEDIOL	non-polymer		62.1	1	Chemie (EDO)
8	F	PENTAETHYLENE GLYCOL	non-polymer		238.3	1	Chemie (1PE)
9	F	TETRAETHYLENE GLYCOL	non-polymer		194.2	1	Chemie (PG4)
10	F	ACETATE ION	non-polymer		59.0	1	Chemie (ACT)
11		water	water		18.0	1390	Chemie (HOH)

Sequence modifications

A, B: 1 - 367 (UniProt: Q51658)

PDB	External Database	Details
Glu 199	Trp 219	engineered mutation
His 368	-	expression tag
His 369	-	expression tag
His 370	-	expression tag
His 371	-	expression tag
His 372	-	expression tag
His 373	-	expression tag

C, E: 1 - 131 (UniProt: A1BBA0)

PDB	External Database	Details
His 132	-	expression tag
His 133	-	expression tag
His 134	-	expression tag
His 135	-	expression tag
His 136	-	expression tag
His 137	-	expression tag

Contents of the asymmetric unit

Polymers	Number of chains	6
	Total formula weight	197128.8
Non-Polymers*	Number of molecules	14
	Total formula weight	3199.7
All*	Total formula weight	200328.6

*Water molecules are not included.